RESUMO A atividade de vôo das abelhas é influenciada por fatores ambientais, como temperatura, umidade e intensidade luminosa; e pelas condições internas da colônia. Informações sobre a atividade externa das abelhas é de grande importância, pois proporciona dados importantes para o conhecimento da biologia das espécies, fornecendo subsídios para o uso destes insetos na polinização de cultivos. O presente trabalho teve como objetivo avaliar a atividade de voo de Geotrigona subterranea (Friese, 1901) (Hymenoptera: Apidae) em ambiente natural. Este estudo foi realizado no Instituto Federal do Norte de Minas Gerais, no município de Januária, Estado de Minas Gerais. Foram observados dois ninhos naturais. A atividade das abelhas foi registrada por três dias a cada mês, durante o período de dezembro de 2011 a novembro de 2012, totalizando 924 observações. Foi registrado o número de abelhas que sai e entra no ninho, e o tipo de material transportado por estas, durante dez minutos a cada hora (das 5 às 19 horas). As abelhas entraram na colónia transportando pólen, resina, detritos e também entraram sem aparente material (néctar ou água). As abelhas iniciaram a atividade externa por volta das 6 horas da manhã, a 20°C e encerraram às 18 horas, a 28,8°C. O pico da atividade de G. subterranea foi observado em torno das 13-14 horas. Apesar de G. subterranea construir seus ninhos em cavidades subterrâneas, suas atividades de forrageamento são semelhantes a de outras espécies de abelhas sem ferrão que constroem ninhos externos ou em cavidades de árvores. Isto indica que, apesar de seu hábito de nidificação, os fatores climáticos afetam seu padrão de forrageamento diária e sazonalmente.

ABSTRACT Flight activity of bees is influenced both by environmental factors and by internal condition of the colonies. Information about external activity of bees is very important, because it provides data of the species biology, supplying subsidies for the use of these insects in the pollination of crops. The present work aim to evaluate the flight activity of Geotrigona subterranea (Friese, 1901) (Hymenoptera: Apidae) in natural environment. This study was performed on the Instituto Federal do Norte de Minas Gerais, in the municipality Januária, Minas Gerais State. Two natural nests were observed. The activities of bees of the colonies were recorded three days each month, during the period of December 2011 to November 2012, totaling 924 observations. It was recorded the number of bees leaving and entering the nest, and the type of material transported by them for ten minutes each hour from 5 a.m. to 7 p.m. The bees entered the colony carrying pollen, resin, detritus and also without apparent material. The bees began external activities by 6 a.m. at 20°C and finished at 6 p.m. at 28.8°C. The peak of activity of G. subterranea occurs on schedule from 1 to 2 p.m. Even though G. subterranea makes their nests in underground, their foraging activities are very similar to others stingless bee species that usually nest on tree cavities or aerial places. This indicate that despite their particular nesting way the external factors as climatic ones will significantly modulate their foraging pattern in a daily and seasonal way.

Due to their high degree of polymorphism, microsatellites are considered useful tools for studying population genetics. Nevertheless, studies of genetic diversity in stingless bees by means of these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under consideration. Herein we compared the number of polymorphic loci and alleles per locus, as well as observed heterozygosity in Melipona rufiventris and M. mondury populations, using specific and heterologous primers. The use of specific primers placed in evidence the greater frequency of polymorphic loci and alleles per locus, besides an expressive increase in observed heterozygosity in M. rufiventris and M. mondury, thereby reinforcing the idea that populational studies should be undertaken by preferably using species-specific microsatellite primers.

The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chromosomes appeared very condensed and presented a high heterochromatic content, making it difficult to localize the centromere and therefore to visualize the chromosomes morphology. The constitutive heterochromatin was located in interstitial chromosome regions covering most of the chromosomes extension and consisted mainly of AT, as shown by DAPI staining. The euchromatin was restricted to the chromosome extremities and was GC-rich, as evidenced by CMA3 staining. The B chromosome was CMA3-negative and DAPI-positive, a heterochromatic constitution similar to that of the A genome chromosomes.

Foram estimadas as correlações entre as características produção de mel, comprimento e largura da tíbia do terceiro par de patas, área corbicular, peso pupal e comprimento da glossa de abelhas africanizadas em Viçosa/MG. Os valores das correlações foram relativamente baixos, embora todos positivos. As características peso pupal e comprimento da glossa foram as que menos se correlacionaram com a produção de mel, 0,225 e 0,410, respectivamente. A área corbicular e o comprimento da tíbia foram as mais correlacionadas com a produção de mel, 0,587 e 0,549, respectivamente. Todas as correlações, exceto o peso pupal, foram significativas. O trabalho dá suporte à idéia de que operárias de maiores áreas corbiculares são mais hábeis a levar mais pólen para suas colméias, sendo possível a obtenção de ganhos na produção de mel, por meio de uma seleção indireta nesta característica.

This study focused on the correlation between honey production length and width of the tibia of the third pair of legs, corbicular area, pupal weight, and glossal length, in Africanized honey bees in Viçosa/MG, Brazil. Correlation values were relatively low, though always positive. The traits glossal length and pupal weight presented the lowest correlation to honey production: 0.225 and 0.410, respectively. The highest correlations were observed between length of the tibia and corbicular area and honey production: 0.587 and 0.549, respectively. In all cases, except pupal weight, correlation was significant. These findings support the notion that worker bees with larger corbicular areas may have a better ability to carry larger quantities of pollen to their hives, so that it is possible to improve honey production through indirect selection of this trait.

Nests of orchid bee Euplusia violacea (Blanchard, 1840) colleted in trap-nests and in the wood of a construction in Viçosa, Minas Gerais (20º45'30"S, 42º52'05"W), Brazil, were studied under laboratory condictions. Some aspects of the biology of these bees were observed, such as sex-ratio, development time, Iongevity and others. Some aspects of the behavior of E. violada were also studied.

Euglossinae bees of two areas of Terra Firme forest, near Manaus, Amazonas, Brazil were studied. During one year the collections were done fortnightly, using traps with eight kinds of scent baits. The traps were placed in the understory and in tree crowns. Some species showed a very clear vertical stratification in the forest. The comparison between the strata studied showed that the fauna of one understory is more similar to other than fauna of crowns and the similarity between fauna of understory and fauna of crown of the the two areaswas low. Some species were specialists while most were generalists in their choice of scent baits. Some species varied its preferences during the year.

The fauna of Euglossinae bees were studied in two areas of "terra firme" continuous forest in the Central Amazon. Brazil. During one year (september/1989-august/1990) the collections were done fortnightly, using traps with eight kinds of seents baits. The traps were placed in the understory and in the tree crowns. The continuous forest of "terra firme" near Manaus are among those with the highest speeies richness of Euglossinae in the Neotropics. The species richness was higher than that found in other studies carried out in Brazil also. One study area had higher species richness and abundance, while species diversity was higher in another one. The diversity was different significally between the two areas.

In Partamona cupira, caste determination follows the general patterns of Trigonini bees. The effects of the juvenile hormone and the amount of food on the process were studied. The frequency of queens increased with the quantity of food ingest by the larvae and or whith the amount of juvenile hormone topically applicated. Queens emerged earlier than workers. Intercastes were not found, sugesting that castes differentiation is a canalised process in this species.